Peptides are not easy to create or produce as the synthetic chemist will tell you. He needs to ensure that amino acids that make up the chain are added in the correct order and that they do not go any other reactions. The process of making or producing synthetic peptide involves adding one amino acid, washing away any unreacted acid then adding the next and it continues. As this can be envisioned, this is a time consuming affair and provides very low yields. A technique that has been relatively developed recently involves attaching one end of the peptide to a solid polymer, meaning that peptide cannot get washed away along with the excess acid. This is quicker than the standard synthesis, and leads to better and improved yields.

Protein expression refers to the way in which proteins are synthesized, modified and regulated in living organisms. We offer a multitude of protein expression systems that are easy to use and deliver high protein yields for every downstream application, as well as custom gene synthesis to optimize cloning and expression. Protein production can occur after gene expression. It consists of the stages after DNA has been transcribed to messenger RNA (mRNA). 

This process of peptide synthesis consists of five steps, carried out in a cyclic fashion.

Step 1 - Attaching an amino acid to the polymer

In the first step of the process initially amino acid is reacted with a molecule known as a "linkage agent" that allows it to attach to a solid polymer, and the other end of the linkage agent is reacted with the polymer support.

Step 2 - Protection

The second step is drawn where an amino acid which is an acid with a basic group at one end and an acid group at the other. To prevent an amino acid from reacting with itself, one of these groups is reacted with something else to make it unreactive.

Step 3 - Coupling

Further ahead, the protected amino acid is then reacted with the amino acid attached to the polymer to begin building the peptide chain.

Step 4 - Deprotection

The protection group is now removed from the acid at the end of the chain so it can react with the next acid which is added later. The new acid is then protected (Step 2) and the cycle continue until a chain of the required length has been synthesized.

Step 5 - Polymer removal

The fifth and last step of peptide synthesis is polymer removal after the desired peptide has been made the bond between the first amino acid and the linkage agent is broken to give the free peptide. Peptides synthesized at Bio Synthesis are widely used in medical research, as they can be synthesized quickly and accurately. When larger amounts of peptides are required they are synthesized in the same manner, and Bio Synthesis is a trusted producer of synthetic peptides for drug and clinical usage in large quantity.